Scientist In The Cell Line Development Group

Skills

• Extensive hands-on experience with aseptic technique and mammalian cell culture for the purpose of generating clonally derived cell lines to produce monoclonal antibodies or vaccine candidates
• Demonstrated ability to produce and screen hundreds of clonally derived CHO or 293 cell lines for top performers
• Expertise in designing and constructing plasmids for gene expression using molecular cloning methods
• Hands-on experience with multiple supporting technologies for cell line engineering, including cell counting, high-throughput transfection, single-cell cloning/deposition, flow cytometry, automated liquid handling systems, and automated small-scale bioreactor systems
• Ability to independently design experiments and work plans and manage project timelines for multiple complex projects simultaneously
• Experience preparing technical reports, SOPs, data presentations, and documents needed for technology transfer and regulatory filing
• Expertise in molecular biology techniques for genetic characterization of cell lines, including RT-PCR and NGS
• Deliverables
• Inspect Equipment — Ad-Hoc
• Meet with lab members to present updates — Ad-Hoc
• Statement of Work Details
• Plan and execute the generation of stable cell lines for preclinical and clinical material production to advance candidates including monoclonal antibodies, recombinant proteins, and virus like particles — 1
• Use molecular biology techniques, including RT-PCR and NGS, for genetic characterization of cell lines — 2
• Gene synthesis including oligo synthesis, plasmid design, and construction — 3
• Independently execute all steps of the process including plasmid construction, stable pool generation, single cell cloning, and top clone selection
• Supports the planning and execution of research experiments in the context of the laboratory's overall research program
• Draft and verify data for CMC sections of pre-IND and IND documents to ensure successful registration of candidates
• Design, perform, and troubleshoot experiments and analyze data for both development and deliverable projects
• Supports laboratory staff on research projects and techniques
• Maintain and support one or more pieces of laboratory instrumentation
• Maintains laboratory equipment and the training of others in their use
• Assists in maintaining adequate space and facilities for ongoing and projected research studies